Chromosome 1 open reading frame 41 Structure

1)Structural similarities

Figure 5: DALI result showing the first 35 proteins that have similar structures to c1orf41. Even though the proteins are similar in structure they have very little sequence identity to c1orf41. 1xpw is c1orf41 but the structure was solved by NMR.

2)Domain Classification

Figure 6: Pfam result indicated that our protein has a F5/8 type C domain, also known as the discoidin domain which is apart of galactose binding domain super family.

3)Protein Structure

Secondary structure

Figure 1: Representation of c1orf41 secondary structure as presented in PDBsum.

Tertiary structure

Figure 2: This protein is monomeric, consisting of a single domain. There are 8 beta strands (yellow) and 2 alpha helices (purple). The secondary structures are folded into jelly roll barrel where four pairs of anti-parallel beta strands are organised to form a barrel-like structure.

Figure 3: Electrostatic charge distribution on the surface of c1orf41.

Figure 4: Surface representation of c1orf41. The Ca (II) ion is located within a pocket of the protein. Sm (III) interacts with Asp92 on the protein but its presence was probably due to the method used in solving the phase problem during structure solution using x-ray crystallogrophy.

4)Possible ligand binding sites

Figure 7: This figure shows the position where calcium (II) ion (yellow) is located in within a loop of c1orf41 structure. This loop where metal ion is coordinated to was also observed in several proteins from the DALI result.

Figure 8:Shown at the top is the discoidin domain of galactose oxidase with a sodium ion (purple) located within a loop. The bottom figure is bacterial sialidase discoidin domain with a sodium ion (purple) and a beta-D-galactose molecule. The sodium ion is also located i within a loop.

Figure 9:CastP identified a surface cleft that is located at the loop position.

Figure 10:Pymol figure displaying possible metal binding position on c1orf41. Pink are the residues that may be involved in coordinating the metal and yellow is the calcium ion. The residues are based on CastP result. The aspartate and glutamate residues are likely to be important in binding positively charged metal ion

Figure 11:Nest 1 and 2 had scores higher than 2. So, they are more likely to be functional. His54 and Lys55 NH atoms are accessible from a large surface cleft of the protein. The cleft is also deep indicating that the nest is functionally important. The residues of Nest 2 are part of the loop that coordinates the Ca (II)ion.

Figure 12:Pymol representation of Nest 1 and 2.

Figure 13:Arrows indicating the pockets of the nests that may be accessible to ligands.

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