Evolutionary analysis of 2ece

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The amino acid sequence of selenium binding protein 2ece, sourced from the organism Sulfolobus tokodaii str. 7, was compared with other proteins using a psi blast(Media:psiblast.txt), with hits from the results hand selected (to show biological diversity) to go through a multiple sequence alignment in clustalx with portions of the alignment shown below

Msatree2ece.png


Msatree2.png


Msatree3.png

The segments in red boxes are reasonably highly conserved amongst the organism, and the segment in a brown box apperas to be a common insertion shared between some organisms. The protein sequences are labelled with gi numbers, the table below associates organisms to specific gi numbers.

Gitable.png

The shown alignment was prepared into a phylogenic tree, shown below, using the nieghbourhood joining method, then creating a consensus tree with bootstrap values. The three kingdoms are circled and labelled and nodes with bootstrap values of less than 75% shown with red dots.

Boottree.png