Fascin Evolution: Difference between revisions

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Sample Fascin sequence (Text File)
Sample Fascin sequence (Text File)


[[Fascin Evolution | Sample Fascin]]
[[Fascin_Evolution | Sample Fascin]]


FASTA sequences from BLAST (Text File)
FASTA sequences from BLAST (Text File)


[[Fascin Evolution | FASTA Seuquences]]
[[Fascin_Evolution | FASTA Seuquences]]


== Multiple Alignment ==
== Multiple Alignment ==

Revision as of 03:52, 9 June 2009

BLAST

Blast Results show that fascin 1 is very highly conserved throughout the Eukarya Domain providing 112 hits all with excellent E value and High scores


Flascin 1.jpg

Sample Fascin sequence (Text File)

Sample Fascin

FASTA sequences from BLAST (Text File)

FASTA Seuquences

Multiple Alignment

From a possible 100 FASTA sequences, were cut down to 31. The process of elimination was conducted by the deletion of sequences with too many bp before and after the sample sequence, followed by the deletion of sequences with large segments of gaps in conserved and variable regions of the sequence alignment. Fascin domains were found via a protDOM search and compared to the remaining sequences.

Fascin multiple alignment.jpg


Phylogency Trees

Neighbor-method with Bootstrap values (1 = 100) Results were run at 500 replications

Fascin bootstrap 500rep.jpg


Neighbor-method with Bootstrap values, compressed. Sequences with below 50 were deleted.

Fascin bootstrap animaliainsecta.jpg