Functional Analysis

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Gaf Domain

New avenues in protein function prediction http://www.proteinscience.org/cgi/content/full/15/6/1527


Sequence -> Function

  • Detecting Protein Function and Protein-Protein Interactions from Genome Sequences

Edward M. Marcotte, Matteo Pellegrini, Ho-Leung Ng, Danny W. Rice, Todd O. Yeates, and David Eisenberg (30 July 1999) Science 285 (5428), 751. [DOI: 10.1126/science.285.5428.751] [1]


Structure -> Function

  • Proknow

Pal D and Eisenberg D. (2005). Inference of protein function from protein structure. Structure 13, 121-130. [2]

Laskowski R A, Watson J D, Thornton J M (2005). ProFunc: a server for predicting protein function from 3D structure. Nucleic Acids Res., 33, W89-W93. [4]

Results:

Locate http://locate.imb.uq.edu.au LOCATE is a curated database that houses data describing the membrane organization and subcellular localization of proteins from the RIKEN FANTOM3 mouse protein sequence set. The membrane organization is predicted by the high-throughput, computational pipeline MemO. The subcellular locations were determined by a high-throughput, immunofluorescence-based assay and by manually reviewing peer-reviewed publications.

2gnx locate.GIF


2gnx BlastP

STRING: a database of predicted functional associations between proteins. String returned no results.

CDART http://www.ncbi.nlm.nih.gov/Structure/lexington/lexington.cgi?cmd=rps - when given a protein query sequence, CDART displays the functional domains that make up the protein and lists proteins with similar domain architectures. Returns no results for mouse sequence.

Pfam didn't work...http://www.sanger.ac.uk/cgi-bin/Pfam/getblast?id=2103981H86007039eB664 - protein families

Profunc http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/GetResults.pl?source=profunc&user_id=ay41&code=073806

Interpro returned no results - protein domain prediction.

Mammalian Orthology http://www.informatics.jax.org/searches/homology_report.cgi?_Marker_key=92352 -- Genes

Mammalian Orthology http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=homologene&dopt=HomoloGene&list_uids=17580 --Proteins

GEGG biochemical pathways

KEGG pathways which involve cAMP


MicroArray Analysis

Entrez gene ID 270802 1. click link to 270802 2. click link to symatlas 3. find correlated

Symatlas is a database full of microarray data for genes in different tissues. It has the function to find correlated microarray data which brings up genes with similar expression data.

The results of a correlation search give a fair few corresponding genes with similar microarray expressions, however the presence of several olfactory receptors seemed interesting : Olfr435, Olfr57, Olfr829, Olfr30.

As the structural team have found a GAF domain on the C-terminal, i tried to find a link between olfactory receptors and the GAF domain. - related to cGMP and PDEs???

Structure of the GAF domain, a ubiquitous signaling motif and a new class of cyclic GMP receptor

cis-regulatory elements

Cis Red - The cisRED database holds conserved sequence motifs identified by genome scale motif discovery, similarity, clustering, co-occurrence and coexpression calculations. regulatory elements in BC048403

Annotation-based Co-occurring Motif Pattern

[5]

aire-foxj2

pax6-pax6

pou1f1-pax6

ipf1-pou1f1

The following table portrays the entire 120 olfactory receptors corresponding to the 14 modules listed accross the top. This table has been cut down to olfactory receptors that have 3 or more co-occuring modules, and is presented in the report.


cdx-2_IPF1 AFP1_POU2F1 AIRE_FOXJ2 cdx-2_foxj2 ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 aire_ipf1 ipf1_pax-6 afp1_pou1f1 afp1_pax-6 afpa_ipf1 pax-6_pax-6 ipf1_pou1f1 count
olfactory receptor 39 [Source:MarkerSymbol;Acc:MGI:1313142] ipf1_pou2f1 1
olfactory receptor 43 [Source:MarkerSymbol;Acc:MGI:1333770] AFP1_POU2F1 pou2f1_pax-6 2
olfactory receptor 58 [Source:MarkerSymbol;Acc:MGI:1333790] AFP1_POU2F1 pou2f1_pax-6 afp1_pax-6 3
olfactory receptor 104 [Source:MarkerSymbol;Acc:MGI:2177487] pou2f1_pax-6 1
olfactory receptor 116 [Source:MarkerSymbol;Acc:MGI:2177499] AIRE_FOXJ2 1
olfactory receptor 123 [Source:MarkerSymbol;Acc:MGI:2177506] ipf1_pou2f1 1
olfactory receptor 127 [Source:MarkerSymbol;Acc:MGI:2177510] pou2f1_pax-6 1
olfactory receptor 135 [Source:MarkerSymbol;Acc:MGI:2177518] afp1_pou1f1 1
olfactory receptor 146 [Source:MarkerSymbol;Acc:MGI:2660711] ipf1_pou2f1 1
olfactory receptor 153 [Source:MarkerSymbol;Acc:MGI:1313138] cdx-2_IPF1 AFP1_POU2F1 ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 afp1_pou1f1 afp1_pax-6 afpa_ipf1 ipf1_pou1f1 9
olfactory receptor 168 [Source:MarkerSymbol;Acc:MGI:3030002] cdx-2_IPF1 1
olfactory receptor 169 [Source:MarkerSymbol;Acc:MGI:3030003] cdx-2_IPF1 AFP1_POU2F1 ipf1_pou2f1 pou2f1_pax-6 afp1_pax-6 ipf1_pou1f1 6
olfactory receptor 170 [Source:MarkerSymbol;Acc:MGI:3030004] ipf1_pou2f1 pou2f1_pax-6 2
olfactory receptor 171 [Source:MarkerSymbol;Acc:MGI:3030005] pou1f1_pax-6 aire_ipf1 ipf1_pax-6 pax-6_pax-6 ipf1_pou1f1 5
olfactory receptor 172 [Source:MarkerSymbol;Acc:MGI:3030006] cdx-2_foxj2
olfactory receptor 178 [Source:MarkerSymbol;Acc:MGI:3030012] ipf1_pou2f1 aire_ipf1 2
olfactory receptor 180 [Source:MarkerSymbol;Acc:MGI:3030014] AIRE_FOXJ2 afp1_pou1f1 2
olfactory receptor 181 [Source:MarkerSymbol;Acc:MGI:3030015] ipf1_pou2f1 pou2f1_pax-6 2
olfactory receptor 183 [Source:MarkerSymbol;Acc:MGI:3030017] ipf1_pou2f1 1
olfactory receptor 192 [Source:MarkerSymbol;Acc:MGI:3030026] ipf1_pou2f1 aire_ipf1 2
olfactory receptor 193 [Source:MarkerSymbol;Acc:MGI:3030027] afp1_pou1f1 1
olfactory receptor 196 [Source:MarkerSymbol;Acc:MGI:3030030] ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 ipf1_pax-6 ipf1_pou1f1 5
olfactory receptor 204 [Source:MarkerSymbol;Acc:MGI:3030038] ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 ipf1_pax-6 4
olfactory receptor 205 [Source:MarkerSymbol;Acc:MGI:3030039] cdx-2_foxj2 ipf1_pou2f1 1
olfactory receptor 295 [Source:MarkerSymbol;Acc:MGI:3030129] ipf1_pou2f1 pou2f1_pax-6 ipf1_pax-6 3
olfactory receptor 303 [Source:MarkerSymbol;Acc:MGI:3030137] pou2f1_pax-6 1
olfactory receptor 310 [Source:MarkerSymbol;Acc:MGI:3030144] cdx-2_IPF1 ipf1_pou2f1 ipf1_pou1f1 3
olfactory receptor 320 [Source:MarkerSymbol;Acc:MGI:3030154] afpa_ipf1 1
olfactory receptor 339 [Source:MarkerSymbol;Acc:MGI:3030173] cdx-2_IPF1 ipf1_pou2f1 2
olfactory receptor 342 [Source:MarkerSymbol;Acc:MGI:3030176] aire_ipf1 1
olfactory receptor 385 [Source:MarkerSymbol;Acc:MGI:3030219] 0 AFP1_POU2F1 afp1_pou1f1 2
olfactory receptor 447 [Source:MarkerSymbol;Acc:MGI:3030281] pou2f1_pax-6 1
olfactory receptor 469 [Source:MarkerSymbol;Acc:MGI:3030303] ipf1_pou1f1 1
olfactory receptor 487 [Source:MarkerSymbol;Acc:MGI:3030321] pou1f1_pax-6 1
olfactory receptor 488 [Source:MarkerSymbol;Acc:MGI:3030322] AFP1_POU2F1 1
olfactory receptor 493 [Source:MarkerSymbol;Acc:MGI:3030327] AFP1_POU2F1 cdx-2_foxj2 ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 afp1_pou1f1 afp1_pax-6 afpa_ipf1 pax-6_pax-6 9
olfactory receptor 494 [Source:MarkerSymbol;Acc:MGI:3030328] ipf1_pou1f1 1
olfactory receptor 495 [Source:MarkerSymbol;Acc:MGI:3030329] cdx-2_IPF1 aire_ipf1 ipf1_pou1f1 3
olfactory receptor 498 [Source:MarkerSymbol;Acc:MGI:3030332] cdx-2_IPF1 ipf1_pou1f1 2
olfactory receptor 502 [Source:MarkerSymbol;Acc:MGI:3030336] cdx-2_IPF1 ipf1_pou2f1 pou2f1_pax-6 aire_ipf1 ipf1_pax-6 pax-6_pax-6 6
olfactory receptor 508 [Source:MarkerSymbol;Acc:MGI:3030342] ipf1_pou2f1 1
olfactory receptor 509 [Source:MarkerSymbol;Acc:MGI:3030343] cdx-2_foxj2 1
olfactory receptor 510 [Source:MarkerSymbol;Acc:MGI:3030344] cdx-2_IPF1 cdx-2_foxj2 ipf1_pou2f1 ipf1_pou1f1 3
olfactory receptor 512 [Source:MarkerSymbol;Acc:MGI:3030346] ipf1_pou2f1 aire_ipf1 2
olfactory receptor 516 [Source:MarkerSymbol;Acc:MGI:3030350] pou1f1_pax-6 1
olfactory receptor 645 [Source:MarkerSymbol;Acc:MGI:3030479] afp1_pou1f1 1
olfactory receptor 666 [Source:MarkerSymbol;Acc:MGI:3030500] afp1_pou1f1 1
olfactory receptor 715 [Source:MarkerSymbol;Acc:MGI:3030549] AFP1_POU2F1 1
olfactory receptor 723 [Source:MarkerSymbol;Acc:MGI:3030557] afp1_pou1f1 afpa_ipf1 ipf1_pou1f1 3
olfactory receptor 724 [Source:MarkerSymbol;Acc:MGI:3030558] ipf1_pou2f1 ipf1_pou1f1 2
olfactory receptor 765 [Source:MarkerSymbol;Acc:MGI:3030599] pou2f1_pax-6 1
olfactory receptor 771 [Source:MarkerSymbol;Acc:MGI:3030605] AIRE_FOXJ2 cdx-2_foxj2 1
olfactory receptor 772 [Source:MarkerSymbol;Acc:MGI:3030606] AIRE_FOXJ2 1
olfactory receptor 774 [Source:MarkerSymbol;Acc:MGI:3030608] cdx-2_foxj2 1
olfactory receptor 777 [Source:MarkerSymbol;Acc:MGI:3030611] cdx-2_IPF1 ipf1_pou1f1 2
olfactory receptor 798 [Source:MarkerSymbol;Acc:MGI:3030632] cdx-2_IPF1 1
olfactory receptor 800 [Source:MarkerSymbol;Acc:MGI:3030634] cdx-2_IPF1 cdx-2_foxj2 ipf1_pou2f1 2
olfactory receptor 801 [Source:MarkerSymbol;Acc:MGI:3030635] ipf1_pou2f1 1
olfactory receptor 803 [Source:MarkerSymbol;Acc:MGI:3030637] AFP1_POU2F1 AIRE_FOXJ2 ipf1_pou2f1 aire_ipf1 4
olfactory receptor 804 [Source:MarkerSymbol;Acc:MGI:3030638] AIRE_FOXJ2 ipf1_pou2f1 aire_ipf1 ipf1_pou1f1 4
olfactory receptor 806 [Source:MarkerSymbol;Acc:MGI:3030640] AIRE_FOXJ2 aire_ipf1 afpa_ipf1 3
olfactory receptor 814 [Source:MarkerSymbol;Acc:MGI:3030648] cdx-2_IPF1 AFP1_POU2F1 cdx-2_foxj2 ipf1_pou2f1 pou2f1_pax-6 ipf1_pax-6 afp1_pax-6 afpa_ipf1 7
olfactory receptor 815 [Source:MarkerSymbol;Acc:MGI:3030649] pou2f1_pax-6 ipf1_pax-6 2
olfactory receptor 816 [Source:MarkerSymbol;Acc:MGI:3030650] ipf1_pou1f1 1
olfactory receptor 821 [Source:MarkerSymbol;Acc:MGI:3030655] ipf1_pou2f1 ipf1_pou1f1 2
olfactory receptor 826 [Source:MarkerSymbol;Acc:MGI:3030660] ipf1_pou2f1 pou1f1_pax-6 ipf1_pax-6 afp1_pou1f1 afp1_pax-6 afpa_ipf1 ipf1_pou1f1 7
olfactory receptor 862 [Source:MarkerSymbol;Acc:MGI:3030696] ipf1_pou1f1 1
olfactory receptor 945 [Source:MarkerSymbol;Acc:MGI:3030779] AIRE_FOXJ2 1
olfactory receptor 951 [Source:MarkerSymbol;Acc:MGI:3030785] ipf1_pou1f1 1
olfactory receptor 965 [Source:MarkerSymbol;Acc:MGI:3030799] AIRE_FOXJ2 1
olfactory receptor 971 [Source:MarkerSymbol;Acc:MGI:3030805] aire_ipf1 afpa_ipf1 2
olfactory receptor 1014 [Source:MarkerSymbol;Acc:MGI:3030848] AFP1_POU2F1 afp1_pou1f1 ipf1_pou1f1 3
olfactory receptor 1029 [Source:MarkerSymbol;Acc:MGI:3030863] pou1f1_pax-6 afp1_pax-6 pax-6_pax-6 0 3
olfactory receptor 1030 [Source:MarkerSymbol;Acc:MGI:3030864] ipf1_pou2f1 aire_ipf1 ipf1_pax-6 3
olfactory receptor 1032 [Source:MarkerSymbol;Acc:MGI:3030866] AFP1_POU2F1 1
olfactory receptor 1036 [Source:MarkerSymbol;Acc:MGI:3030870] AFP1_POU2F1 1
olfactory receptor 1071 [Source:MarkerSymbol;Acc:MGI:3030905] pou1f1_pax-6 pou2f1_pax-6 2
olfactory receptor 1094 [Source:MarkerSymbol;Acc:MGI:3030928] AIRE_FOXJ2 ipf1_pou2f1 2
olfactory receptor 1115 [Source:MarkerSymbol;Acc:MGI:3030949] AFP1_POU2F1 1
olfactory receptor 1116 [Source:MarkerSymbol;Acc:MGI:3030950] cdx-2_IPF1 AFP1_POU2F1 ipf1_pou2f1 3
olfactory receptor 1121 [Source:MarkerSymbol;Acc:MGI:3030955] cdx-2_IPF1 ipf1_pou2f1 2
olfactory receptor 1123 [Source:MarkerSymbol;Acc:MGI:3030957] ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 ipf1_pax-6 ipf1_pou1f1 5
olfactory receptor 1126 [Source:MarkerSymbol;Acc:MGI:3030960] cdx-2_foxj2
olfactory receptor 1129 [Source:MarkerSymbol;Acc:MGI:3030963] cdx-2_IPF1 ipf1_pou1f1 2
olfactory receptor 1130 [Source:MarkerSymbol;Acc:MGI:3030964] cdx-2_IPF1 ipf1_pou1f1 2
olfactory receptor 1131 [Source:MarkerSymbol;Acc:MGI:3030965] pou1f1_pax-6 pou2f1_pax-6 2
olfactory receptor 1140 [Source:MarkerSymbol;Acc:MGI:3030974] ipf1_pou2f1 ipf1_pou1f1 2
olfactory receptor 1145 [Source:MarkerSymbol;Acc:MGI:3030979] AFP1_POU2F1 ipf1_pou2f1 2
olfactory receptor 1148 [Source:MarkerSymbol;Acc:MGI:3030982] pou1f1_pax-6 afp1_pou1f1 2
olfactory receptor 1151 [Source:MarkerSymbol;Acc:MGI:3030985] AFP1_POU2F1 ipf1_pou2f1 afp1_pou1f1 ipf1_pou1f1 4
olfactory receptor 1152 [Source:MarkerSymbol;Acc:MGI:3030986] ipf1_pou2f1 pou1f1_pax-6 ipf1_pax-6 ipf1_pou1f1 4
olfactory receptor 1153 [Source:MarkerSymbol;Acc:MGI:3030987] cdx-2_IPF1 ipf1_pou2f1 afpa_ipf1 ipf1_pou1f1 4
olfactory receptor 1161 [Source:MarkerSymbol;Acc:MGI:3030995] cdx-2_IPF1 pou1f1_pax-6 aire_ipf1 ipf1_pax-6 afp1_pou1f1 afpa_ipf1 ipf1_pou1f1 7
olfactory receptor 1178 [Source:MarkerSymbol;Acc:MGI:3031012] AFP1_POU2F1 afp1_pou1f1 2
olfactory receptor 1184 [Source:MarkerSymbol;Acc:MGI:3031018] cdx-2_IPF1 1
olfactory receptor 1189 [Source:MarkerSymbol;Acc:MGI:3031023] ipf1_pax-6 1
olfactory receptor 1193 [Source:MarkerSymbol;Acc:MGI:3031027] cdx-2_IPF1 AIRE_FOXJ2 2
olfactory receptor 1202 [Source:MarkerSymbol;Acc:MGI:3031036] afpa_ipf1 0 ipf1_pou1f1 2
olfactory receptor 1203 [Source:MarkerSymbol;Acc:MGI:3031037] 0 AFP1_POU2F1 1
olfactory receptor 1257 [Source:MarkerSymbol;Acc:MGI:3031091] ipf1_pou2f1 pou2f1_pax-6 ipf1_pax-6 ipf1_pou1f1 4
olfactory receptor 1259 [Source:MarkerSymbol;Acc:MGI:3031093] ipf1_pou1f1 1
olfactory receptor 1276 [Source:MarkerSymbol;Acc:MGI:3031110] ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 ipf1_pax-6 ipf1_pou1f1 5
olfactory receptor 1278 [Source:MarkerSymbol;Acc:MGI:3031112] cdx-2_foxj2 ipf1_pou1f1 1
olfactory receptor 1279 [Source:MarkerSymbol;Acc:MGI:3031113] ipf1_pou1f1 1
olfactory receptor 1283 [Source:MarkerSymbol;Acc:MGI:3031117] ipf1_pou1f1 1
olfactory receptor 1287 [Source:MarkerSymbol;Acc:MGI:3031121] ipf1_pou1f1 1
olfactory receptor 1302 [Source:MarkerSymbol;Acc:MGI:3031136] cdx-2_foxj2
olfactory receptor 1362 [Source:MarkerSymbol;Acc:MGI:3031196] afp1_pou1f1 1
olfactory receptor 1406 [Source:MarkerSymbol;Acc:MGI:3031240] pou2f1_pax-6 1
olfactory receptor 1426 [Source:MarkerSymbol;Acc:MGI:3031260] ipf1_pou1f1 1
olfactory receptor 1436 [Source:MarkerSymbol;Acc:MGI:3031270] cdx-2_IPF1 1
olfactory receptor 1448 [Source:MarkerSymbol;Acc:MGI:3031282] cdx-2_foxj2 pou2f1_pax-6 pax-6_pax-6 2
olfactory receptor 1451 [Source:MarkerSymbol;Acc:MGI:3031285] afp1_pou1f1 1
olfactory receptor 1457 [Source:MarkerSymbol;Acc:MGI:3031291] ipf1_pou2f1 pou1f1_pax-6 pou2f1_pax-6 ipf1_pax-6 ipf1_pou1f1 5
olfactory receptor 1480 [Source:MarkerSymbol;Acc:MGI:3031314] ipf1_pou2f1 1
olfactory receptor 1495 [Source:MarkerSymbol;Acc:MGI:3031329] afp1_pax-6 1
olfactory receptor 1508 [Source:MarkerSymbol;Acc:MGI:3031342] AIRE_FOXJ2 1
olfactory receptor 1510 [Source:MarkerSymbol;Acc:MGI:3031344] pou2f1_pax-6 1
Olfactory receptor MOR218-3. [Source:Uniprot/SPTREMBL;Acc:Q8VF67] AFP1_POU2F1 1
22 20 12 119 43 17 27 13 16 17 8 11 5 38 250


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