HAD

• HAD (Haloacid dehalogenase-like) family
  • Phosphatase activity: CO–P bond hydrolysis
  • Dehalogenase activity: C–halogen bond hydrolysis
  • Phosphonatase: C–P bond hydrolysis
  • Phosphoglucomutase: CO–P bond hydrolysis and intramolecular phosphoryl transfer

• HAD family
  • Dependent on the presence of Mg²⁺
  • Inhibited by vanadate and Ca²⁺

• E. coli genome encodes
  • 23 soluble HAD-like hydrolases
  • Representing about 40% of the E. coli
  • Metabolites are intermediates of various metabolic pathways and reactions
  • Glycolysis, pentose phosphate pathway, gluconeogenesis, and intermediary sugar and nucleotide metabolism