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Structural, functional and evolutionary analyses collectively indicate that Nucleotide Binding Protein 2 (MinD homolog, E.coli) is one of the membrane associated ATPase family. It involved in cell division to determine the position of septum (Z-ring) though the MinD complex. NUBP2 is the paralog to prokaryotic MRP proteins; converged with bacterial MinD; and orginated from NUBP1.