Phostphatase

Phosphatase

MSA of the 2gfh with 35 others proteins. Only the 60^th – 70^th and the 210^th -300^th amino acid

sequence were shown to illustrate the conserved and invariant regions. The 3 boxed-up sequences were either conserved or invariant regions.

• 1st - aspartic acid (D)

• 2nd - threonine (T), asparagine (N) and glycine (G)

• 3rd - lysine (K) and aspartates (D)

MSA corelate with with study done by Maliekal et al (2006)

Alignment of rat and human Neu5Ac-9-P phosphatase with homologous sequences. The following sequences are aligned: Rattus

norvegicus (Rnor, gi-34859431), Homo sapiens (Hsap, gi-23308749), Xenopus laevis (Xlae, gi-46250196), Danio rerio (Drer, gi-

63101958), and Drosophila melanogaster (Dmel, gi-28381565). Only the first 280 residues of the latter sequence are shown. Completely

conserved residues are shown in boldface type. Asterisks indicate the extremely conserved residues in phosphatases of the HAD family ⁸.

• N-acetylneuraminic acid phosphatase orthologs shared 3 motifs found in phosphatases
  • 1^st motif comprising two extremely conserved aspartates (D)
  • 2^nd motif comprising a conserved serine (S) or threonine (T)
  • 3^rd motif comprising a conserved lysine (K) and two conserved aspartates (D)

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