Phostphatase: Difference between revisions

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* MSA corelate with with study done by Maliekal et al  
* MSA corelate with with study done by Maliekal et al  
**N-acetylneuraminic acid phosphatase orthologs shared 3 motifs found in phosphatases
 


[[Image:Document19_08.png]]
[[Image:Document19_08.png]]


'''Figure 19. ''' Alignment of rat and human Neu5Ac-9-P phosphatase with homologous sequences. The following sequences are aligned: ''Rattus  
Alignment of rat and human Neu5Ac-9-P phosphatase with homologous sequences. The following sequences are aligned: ''Rattus  


norvegicus'' (Rnor, gi-34859431), ''Homo sapiens'' (Hsap, gi-23308749), ''Xenopus laevis'' (Xlae, gi-46250196), ''Danio rerio'' (Drer, gi-
norvegicus'' (Rnor, gi-34859431), ''Homo sapiens'' (Hsap, gi-23308749), ''Xenopus laevis'' (Xlae, gi-46250196), ''Danio rerio'' (Drer, gi-
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The MSA done by Maliekal ''et al'' shows that the Neu5Ac-9-Pase orthologs shared the three motifs found in phosphatases of the HAD family,
*N-acetylneuraminic acid phosphatase orthologs shared 3 motifs found in phosphatases  
 
**1<sup>st</sup> motif comprising two extremely conserved aspartates (D)
namely a 1<sup>st</sup> motif comprising two extremely conserved aspartates (D), a 2<sup>nd</sup> motif comprising a conserved serine (S) or  
**2<sup>nd</sup> motif comprising a conserved serine (S) or threonine (T)
 
**3<sup>rd</sup> motif comprising a conserved lysine (K) and two conserved aspartates (D)
threonine (T), and a 3<sup>rd</sup> motif comprising a conserved lysine (K) and two conserved aspartates (D) <sup>8</sup>. The first aspartate
 
in the first motif forms a phosphoaspartate during the catalytic cycle <sup>9</sup>. These findings suggested therefore that the HDHD4 protein


was a phosphatase. The first aspartate in the first motif forms a phosphoaspartate during the catalytic cycle <sup>10</sup>. In our MSA (Figure


16), the several conserved motifs that shared great similarity to the study done by Maliekal ''et al''. These findings suggested therefore that


Neu5Ac-9-P phosphatase protein is a phosphatase
*HAD (Haloacid dehalogenase-like) family
** Phosphatase activity:  CO–P bond hydrolysis
** Dehalogenase activity:  C–halogen bond hydrolysis
** Phosphonatase:          C–P bond hydrolysis
** Phosphoglucomutase:    CO–P bond hydrolysis and intramolecular phosphoryl transfer




[[Role in Human|Next Page]]
[[Role in Human|Next Page]]

Revision as of 01:27, 12 June 2007

Phosphatase

Document9 01.png

MSA of the 2gfh with 35 others proteins. Only the 60th – 70th and the 210th -300th amino acid

sequence were shown to illustrate the conserved and invariant regions. The 3 boxed-up sequences were either conserved or invariant regions.


  • 1st - aspartic acid (D)
  • 2nd - threonine (T), asparagine (N) and glycine (G)
  • 3rd - lysine (K) and aspartates (D)


  • MSA corelate with with study done by Maliekal et al


Document19 08.png

Alignment of rat and human Neu5Ac-9-P phosphatase with homologous sequences. The following sequences are aligned: Rattus

norvegicus (Rnor, gi-34859431), Homo sapiens (Hsap, gi-23308749), Xenopus laevis (Xlae, gi-46250196), Danio rerio (Drer, gi-

63101958), and Drosophila melanogaster (Dmel, gi-28381565). Only the first 280 residues of the latter sequence are shown. Completely

conserved residues are shown in boldface type. Asterisks indicate the extremely conserved residues in phosphatases of the HAD family 8.


  • N-acetylneuraminic acid phosphatase orthologs shared 3 motifs found in phosphatases
    • 1st motif comprising two extremely conserved aspartates (D)
    • 2nd motif comprising a conserved serine (S) or threonine (T)
    • 3rd motif comprising a conserved lysine (K) and two conserved aspartates (D)



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