View of the Binding Pocket
The large cavity of the 2nxf protein shown in a surface representation (left), showing its relative position with the rest of the protein; the ligands are shown here in red. The ribbon view (right) shows that the ligands are positioned at the mid-point sandwiched between the two halves of the protein, above the inner beta-sheet centre of the protein. The ribbon view, with mesh surface superimposed on top (bottom) is a close up of this same binding pocket, viewed from the right side of the protein. Analysis of the Dali results showed that the top 5 sequences contained three unchanging amino acid residues at positions 95 to 97 with a highly conserved acidic amino acid at position 98. The side-chains of conserved residues are shown here as coloured sticks. The two Zn atoms (spheres) appear to be indented into the surface of the protein with the phosphorous ion ligand sitting just above them on the surface of the protein.
Ligand Interactions
Below are the interactions between the two Zn atoms and the PO4 ion in the binding pocket. Some of the residues shown here are conserved or invariant, such as Asn96, His97 and His265.
