Investigation into the human fascin-1 protein was undertaken to understand its evolutionary history as well as its structural & functional properties. High sequence identity was found across multiple different eukaryotic species. Structuraly similar proteins were found to have very little sequence identity and no functional relationship to actin-binding protein fascin.
The two actin-binding regions of fascin were narrowed down to the the N-terminal region and C-terminal region of fascin which is conserved throughout different species studied to date. Although exact binding region is still unknown, we present that it encompasses residues 136-143 and 386-395 at the N and C terminals respectively.